Reconstructing Fragmented Gel Files Created by Model 377 DNA Sequencers

Reconstructing fragmented gel files created by Model 377 DNA Sequencers.

To our knowledge, the most widely used automated DNA sequencers are manufactured by PE Applied Biosystems (Foster City, CA, USA). Our laboratory uses Model 377 Sequencers equipped with the XL upgrade (i.e., collection software Version 2.0 and analysis software Version 3.0). Power transients, network noise, an excessively fragmented hard disk or other such incidents can cause a sequencer to prem...

Correcting data shifts in gel files created by Model 377 DNA Sequencers.

Rescuing corrupted gel files from Model 377 and 373 DNA Sequencers.

Automated DNA sequencing requires the intensive use of computers to handle the large amount of data taken. When a computer failure occurs and the data are no longer accessible, all the expense and effort that went into the sequencing experiment is lost. By using the data storage architecture of Macintosh computers to our advantage, we may prevent this loss in the case of automatic sequencers fr...

Reconstructing Fragmented YAFFS2 Files for Forensic Analysis

Data recovery from captured intelligent mobile devices such as smartphones plays a significant role in digital forensic analysis. In this paper, we study the main characteristics of NAND flash and YAFFS2 file systems and explore the method for recovering YAFFS2 files for forensic analysis based on Tnode tree that can save a lot of time compared to other data recovery methods. For any broken fil...

Improving read lengths by recomputing the matrices of Model 377 DNA Sequencers.

To our knowledge, the most widely used automated DNA sequencers in the world are the Model 377 and Model 373 DNA Sequencers manufactured by PE Applied Biosystems (Foster City, CA, USA). These sequencers detect the emission spectra of the four fluorescent dyes in sequencing reactions that are excited by an argon ion laser. Each of the four possible bases are labeled by a different dye, possessin...